- HCM014 Triple Sugar Iron Agar (TSI)
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CATEGORY :
Dehydrated Culture Media
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USAGE :
For differentiating Gram-negative enteric bacilli.
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SPECIFICATION :
500g/bottle
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CODE :
HCM014
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HCM014 Triple Sugar Iron Agar (TSI)
Dehydrated Culture Media
Usages:
For differentiating Gram-negative enteric bacilli.
Principle:
Peptone, beef extract powder provide nitrogen, vitamins, minerals; lactose, dextrose, sucrose to fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.
Formulation (per liter):
Pancreatic Digest of Casein 10g
Pancreatic Digest of Animal Tissue 10g
Lactose 10g
Sucrose 10g
Dextrose 1g
Ferrous Ammonium Sulfate 200mg
Sodium Chloride 5g
Sodium Thiosulfate 200mg
Agar 13g
Phenol Red 25mg
Final pH 7.3±02
How to use:
1.Suspend 59.4g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool a slanted position.
2.Diluted and treated samples.
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 500g/bottle
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