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HCM009 Rappaport Vassiliadis Salmonella Enrichment Broth (RVS)

CATEGORY :

Dehydrated Culture Media

USAGE :

For selective enrichment of Salmonella.

SPECIFICATION :

500g/bottle

CODE :

HCM009

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  • MSDS
HCM009 Rappaport Vassiliadis Salmonella Enrichment Broth (RVS)
 
Usages:
For selective enrichment of Salmonella.
 
Principle:
Tryptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; potassium dihydrogen phosphate is buffers; magnesium chloride medium increased osmotic pressure; malachite green suppressing non salmonella bacteria; lower The combination of magnesium chloride and malachite green pH culture medium with high selectivity.
 
Formulation(per liter):
Soy Peptone: 4.5g
Sodium Chloride: 8.0g
Potassium Dihydrogen Phosphate: 0.6g
Dipotassium Hydrogen Phosphate :0.4g
Magnesium Chloride anhydrous :13.58g
Malachite Green :0.036g
Final pH 5.2 ± 0.2          
 
How to use:
1.Suspend 27.1g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
 
Quality control:
            
Item The name and number of strain Growth Colony Color
1 Salmonella typhimurium CMCC (B) 50115 Good Cloudy broth
2 Paratyphoid Salmonella CMCC (B) Good Cloudy broth
3 Escherichia coli ATCC25922 Not growth --
 
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
 
Specifications: 500g/bottle