- HCM006 Violet Red Bile Glucose Agar (VRBGA)
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CATEGORY :
Dehydrated Culture Media
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USAGE :
For the presumptive enumeration of Enterobacteriaceae.
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SPECIFICATION :
500g/bottle
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CODE :
HCM006
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HCM 006 Violet Red Bile Glucose Agar (VRBGA)
Usages:
For selective isolation of Enterobacter sakazakii and differentiating of enterobacteriaceae.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.
Formulation（per liter）:
Pancreatic Digest of Gelatin: 7g
Yeast extract powder: 3g
Lactose: 10g
Glucose Monohydrate: 10g
Sodium chloride: 5g
The 3rd bile: 1.5g
Neutral Red :0.03g
Crystal violet: 0.002g
Agar: 15g
Final pH 7.4 ± 0.2
How to use：
1.Suspend 41.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, autoclave at 121 ℃ for 15min.
2.Diluted and treated samples.
Quality control：
|
Item |
The name and number of strain |
Growth |
Colony Color |
|
1 |
Escherichia coli ATCC25922 |
Good |
Dark red |
|
2 |
Aerogenes CMCC (B) 45103 |
Good |
red |
|
3 |
Staphylococcus aureus ATCC6538 |
Suppressed |
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Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle
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FOCUS ON MICROBIAL DETECTION & CONTROL
