- HCM045 Mannitol Salt Agar
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CATEGORY :
Dehydrated Culture Media
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USAGE :
For selective isolation and culture of Staphylococcus aureus.
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SPECIFICATION :
500g/bottle
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CODE :
HCM045
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HCM045 Mannitol Salt Agar
Usages:
For selective isolation and culture of Staphylococcus aureus.
Principle:
Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
Formulation（per liter）:
Pancreatic digest of casein 5.0g
Pancreatic digest of animal tissue 5.0g
Beef Extract 1.0g
Sodium Chloride 75.0g
Mannitol 10.0g
Phenol Red 0.025g
Agar 15.0g
Final PH 7.4±0.2
How to use：
1.Suspend 111g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control：
|
Item |
The name and number of strain |
Growth |
Colony Color |
|
1 |
Staphylococcus aureus CMCC (B) 26003 |
Good |
Golden yellow |
|
2 |
Staphylococcus epidermidis CMCC (B) 26069 |
Good |
Red |
|
3 |
Escherichia coli CMCC (B) 44102 |
Inhibition |
-- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle
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FOCUS ON MICROBIAL DETECTION & CONTROL
